Journal: Journal of Lipid Research

Article Title: Epigenetic control of microsomal prostaglandin E synthase-1 by HDAC-mediated recruitment of p300

doi: 10.1194/jlr.M072280

Figure Lengend Snippet: PTGES1 expression is reduced by HDACi (A, B) or depletion (C). A–C: qRT-PCR of the genes indicated (relative to β-actin) from murine carotid rings (A) and from human VSMCs (B, C). A: Effect of SAHA (2 μmol/l, 14 h). B: Effect of SAHA or apicidin (14 h), both relative to the control DMSO (Ctl). C: Effect of two different control scrambled RNAi (siSCRs) or siRNA against HDAC as indicated. *P < 0.05, n ≥ 3.

Article Snippet: Human VSMCs [human aortic smooth muscle cells #354-05a, human coronary artery smooth muscle cells #350-05a, and human carotid smooth muscle cells #3514-05a] were purchased from PELOBiotech (Planegg, Germany).

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: eNeuro

Article Title: Pericyte-Mediated Tissue Repair through PDGFRβ Promotes Peri-Infarct Astrogliosis, Oligodendrogenesis, and Functional Recovery after Acute Ischemic Stroke

doi: 10.1523/ENEURO.0474-19.2020

Figure Lengend Snippet: Leptomeningeal arteriogenesis and recovery of CBF in ischemic areas after pMCAO are attenuated in Pdgfrb +/– mice. A , Images of CBF just under the cortical surface, as assessed by laser speckle flowmetry, in control and on days 1, 7, 14, and 28 after pMCAO in wild-type and Pdgfrb +/– mice (scale bar, 2 mm). B , Temporal profiles of CBF quantification on days 1, 7, 14, and 28 after pMCAO ( n = 12, each group). C , Macroscopic images of leptomeningeal anastomoses, assessed by a latex perfusion method, at baseline ( a , c ) and on day 7 ( b , d ) after pMCAO in wild-type and Pdgfrb +/– mice (scale bar, 50 μm). Arrowheads indicate the leptomeningeal anastomoses vessels ( b , d ). Magnified images of the dotted squares ( e – h ) in a – d are shown in the right panels. Cortical whole mount immunostaining of leptomeningeal anastomoses with CD31 (red) on day 7 after pMCAO is shown in i (a: artery, v: vein). Arrows indicate the pre-existing ACA and MCA. The magnified image of the yellow dotted square ( j ) is shown in the right panel. Double immunofluorescence labeling with CD31 (red) and αSMA (green) in anastomosed vessels on day 7 after pMCAO in wild-type ( j ) and Pdgfrb +/– mice ( k ) is shown (scale bar, 50 μm). D , Diameter of anastomotic vessels at baseline and on day 7 after pMCAO (baseline, n = 7 each group; pMCAO, n = 8 each group). E , Number of anastomotic vessels at baseline and on day 7 after pMCAO (baseline, n = 7 each group; pMCAO, n = 8 each group). F , mRNA levels of arteriogenesis-related genes in non-infarct hemisphere and ischemic hemisphere on day 7 ( n = 8, each group). G , Cortical whole mount immunostaining with CD31 (red), F4/80 (green), and DAPI (blue) in wild-type and Pdgfrb +/– mice (scale bar, 50 μm). H , Quantitative PCR for Ccl2 / Mcp1 and Tnfa in cultured VSMCs at baseline (black), with PDGF-BB (10 ng/ml; red), and with SU16f-pretreated PDGF-BB (100 nmol/l; blue; n = 6, each group). Data are shown as the mean ± SEM. B , F , † p < 0.1, * p < 0.05, ** p < 0.01, and *** p < 0.001, unpaired t test. D – E , H , † p < 0.1, * p < 0.05, ** p < 0.01, and *** p < 0.001, one-way ANOVA followed by Bonferroni’s post hoc test.

Article Snippet: Human brain vascular smooth muscle cells (SMCs) were purchased from ScienCell Research and Laboratories and cultured in SMC medium containing 2% fetal bovine serum (FBS) and SMC growth supplement.

Techniques: Control, Immunostaining, Immunofluorescence, Labeling, Real-time Polymerase Chain Reaction, Cell Culture

Journal: bioRxiv

Article Title: Mitochondria-targeted hydrogen sulfide donor reduces atherogenesis by reprogramming macrophages and increasing UCP1 expression in vascular smooth muscle cells

doi: 10.1101/2024.05.15.594319

Figure Lengend Snippet: Representative immunohistochemical staining of aortic roots showing calponin (green), DAPI (blue) and UCP1 (red) co-localization in control and AP39-treated mice (A) . White arrows indicate some of the UCP1 & calponin positive cells. Quantitative analysis of UCP1 expression in VSMCs in atherosclerotic lesions (B) . Mean ± SEM; **p<0.01 compared to control mice; n=13; unpaired two-tailed Student’s t -test.

Article Snippet: To evaluate uncoupling protein 1 (UCP1) expression in VSMCs, antibodies against the marker of VSMCs - calponin (dilution 1:200, Proteintech, USA) and UCP1 (dilution 1:200, Thermo Scientific USA) were used.

Techniques: Immunohistochemical staining, Staining, Control, Expressing, Two Tailed Test